Phospho-mTOR (Ser2448) rabbit mAb Antibody

SKU: orb1946060

Description

mTOR, m ammalian t arget o f r apamycin, is a Serine/Threonine protein kinase (1-2) that functions as an amino acid and ATP sensor to balance cell growth and nutrient availability (3-4). When sufficient nutrients are available, mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of 4E-BP1 (5). mTOR plays a key role in homeostasis and cell growth, and phospho mTOR may be abnormally regulated in tumors. mTOR is a potential target for anti-cancer therapy (6).

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: BG33
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.01 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or treated with PDGF (green) using Phospho-mTOR (Ser2448) antibody mTORS2448-E11 at 0.01 μg/mL. Cat. #orb1946060.

Quick Database Links

UniProt

P42345