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Catalog Number | orb18232 |
---|---|
Category | Antibodies |
Description | Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | MAPK-YT |
Tested applications | ICC, IHC, WB |
Predicted Reactivity | Bovine, Monkey |
Reactivity | Human, Mouse, Rat, Yeast |
Isotype | Mouse IgG1 |
Immunogen | A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH. |
Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat, yeast, By Heat Immunocytochemistry , 1μg/ml, Human, mouse, rat, yeast, -Western blot, 0.25-0.5μg/ml, Human, mouse, rat, yeast |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 43081 MW |
UniProt ID | P21708 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Mitogen-activated protein kinase 3;MAP kinase 3;MA Read more... |
Note | For research use only |
Application notes | Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of MAP kinase;activated using anti-MAP kinase;activated antibody.Lane 1:human K562 cell.
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