Phospho-JUN Antibody (pT91/93)

• Catalog Number: orb2642509
• Category: Antibodies
• Description: This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [RefSeq]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: The amino acids surrounding phosphorylated T91 and T93 were used as the immunogen for the phospho-c-Jun antibody.
• UniProt ID: P05412
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Optimal dilution of the phospho-c-Jun antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: C-J 4C4/1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This phospho-c-Jun antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human cervical tumor with phospho-c-Jun antibody (clone C-J 4C4/1). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human cervical tumor with phospho-c-Jun antibody (clone C-J 4C4/1). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free phospho-c-Jun antibody (clone C-J 4C4/1) as confirmation of integrity and purity.