Phospho-IKB alpha (Ser32) Recombinant Rabbit Monoclonal Antibody

SKU: orb559185

Description

Phospho-IKB alpha (Ser32) Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, WB
• Dilution range: WB=1:500-1000, ICC/IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 13E7
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IKB alpha around the phosphorylation site of Ser32 D(p-S)GL
• Target: NFKBIA
• Molecular Weight: 36 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

IKB alpha(S32); IKB alpha(phospho S32); IKB alpha(phospho Ser32); p-IKB alpha(phospho S32); I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; IKBA_HUMAN; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-phospho-IKB alpha (Ser32) antibody (orb559185), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-IKB alpha (S32)) monoclonal Antibody, Unconjugated (orb559185) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: SK-Br-3 cell lysates, Primary: Anti-phospho-IKB alpha (Ser32) (orb559185) at 1/500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.

Quick Database Links

Gene Symbol

NFKBIA

UniProt

P25963