Phospho-eIF4E (Ser209) Antibody

• Catalog Number: orb1733095
• Category: Antibodies
• Description: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E
• Concentration: 1mg/ml
• Dilution range: IP: 1/20; WB: 1/500-1/1000; IHC: 1/50-1/100; ICC: 1/50-1/200
• Conjugation: Unconjugated
• MW: Calculated MW: 25 kDa; Observed MW: 25 kDa
• Entrez: 1977
• UniProt ID: P06730
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: EIF4E; EIF4EL1; EIF4F; Eukaryotic translation init
• Note: For research use only
• Expiration Date: 12 months from date of receipt.