Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (CC12) rabbit mAb

• Catalog Number: orb1946052
• Category: Antibodies
• Description: Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (CC12) rabbit mAb
• Clonality: Monoclonal
• Clone Number: AuroraABC-CC12
• Tested applications: FC
• Reactivity: Human
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding human Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: O14965, Q9UQB9, Q96GD4
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Aurora kinase A/B/C, AURKA, AURKB, AURKC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (grey) or treated with nocodazole (orange) using 0.5 μg/mL of isotype control Cat. #orb1946241 or untreated (red) or treated (green) using Phospho-Aurora A (Thr288) /Aurora B (Thr232) /Aurora C (Thr198) antibody AuroraABC-CC12 at 0.5 μg/mL. Cat. #orb1946052.

Peptide blocking flow cytometric analysis of HeLa cells unstained cells negative control (light blue) or untreated (red) or treated with nocodazole (green) or untreated and blocked with phospho-peptide (black) or nocodazole and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or nocodazole and blocked with non-phospho peptide (purple) using Phospho-Aurora A (Thr288) /Aurora B (Thr232) /Aurora C (Thr198) antibody AuroraABC-CC12 at 0.5 μg/mL. Cat. #orb1946052.