Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (CC12) rabbit mAb Antibody

SKU: orb1946052

Description

Aurora kinases (serine/threonine kinases) are essential requirement for the onset and progression of mitosis. These kinases share a similar protein structure as well as kinase activity, however each kinase display distinct cellular and subcellular localization. Each Aurora member is phosphorylated at specific residues upon co-factor binding during mitosis. Aurora kinases acquire active kinase conformations due to the activation loop. The active kinase conformation is acquired upon auto-phosphorylation through an intermolecular (trans)-reaction within Aurora kinase domain. Aurora Kinase A (Aurora A) is involved in G2/M transition. AuroraA promotes centrosome maturation and mitotic spindle assembly, whereas AuroraB and AuroraC act as chromosome-passenger complex proteins. They play a crucial role in chromosomal binding to kinetochores and segregation of chromosomes. Aurora B is widely distributed in the cell, while AuroraC is expressed mainly in the meiotically-active germ cells. Aurora kinases are auto-phosphorylated into active forms at conserved threonine residues (i.e. the Thr288 (AurA), Thr232 (AurB) and Thr195 (AurC) residues). AuroraA auto-phosphorylation is initiated by several co-factors acting at different steps of mitosis. AroraB and AruroaC auto-phosphorylation are mediated by survivin and Borealin proteins.

Images & Validation

• Tested Applications: FC
• Dilution range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: AuroraABC-CC12
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding human Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Alternative Names

Aurora kinase A/B/C, AURKA, AURKB, AURKC

Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (grey) or treated with nocodazole (orange) using 0.5 μg/mL of isotype control Cat. #orb1946241 or untreated (red) or treated (green) using Phospho-Aurora A (Thr288) /Aurora B (Thr232) /Aurora C (Thr198) antibody AuroraABC-CC12 at 0.5 μg/mL. Cat. #orb1946052.

Peptide blocking flow cytometric analysis of HeLa cells unstained cells negative control (light blue) or untreated (red) or treated with nocodazole (green) or untreated and blocked with phospho-peptide (black) or nocodazole and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or nocodazole and blocked with non-phospho peptide (purple) using Phospho-Aurora A (Thr288) /Aurora B (Thr232) /Aurora C (Thr198) antibody AuroraABC-CC12 at 0.5 μg/mL. Cat. #orb1946052.

Quick Database Links

UniProt

Q96GD4, O14965, Q9UQB9