Phospho-Ask1 (Ser83) (G4) rabbit mAb

• Catalog Number: orb1946273
• Category: Antibodies
• Description: Phospho-Ask1 (Ser83) (G4) rabbit mAb
• Clonality: Monoclonal
• Clone Number: Ask1S83-G4
• Tested applications: FC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser83 of human phospho Ask1
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q99683
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Apoptosis signal-regulating kinase 1, Mitogen-acti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with IFNα + IL-4 + pervanadate (green) using Phospho-Ask1 (Ser83) antibody Ask1S83-G4 at 5ng/mL. Cat. #orb1946273.

Western blot analysis of L929 cell extract treated with 20% FBS using Phospho-Ask1 (Ser83) antibody Ask1S83-G4 at 0.01μg/mL. Cat. #orb1946273.

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or imatinib-treated (red) or pervandadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-Ask1 (Ser83) antibody Ask1S83-G4 at 1ng/mL. Cat. #orb1946273.

Western blot analysis of C6 cell extract treated with imatinib or treated with PV using Phospho-Ask1 (Ser83) antibody at 0.01 μg/mL. Ask1S83-G4. Cat. #orb1946273.

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-Ask1 (Ser83) antibody Ask1S83-G4 at 1ng/mL. Cat. #orb1946273.

Western blot analysis of C6 cell extract treated with imatinib or with pervanadate using 0.01 μg/mL Phospho-Ask1 (Ser83) antibody Ask1S83-G4 Cat. #orb1946273 or Company C antibody at 0.2 μg/mL (manufacturer's recommended concentration) developed using the same exposure.