PHF8 Antibody

• Catalog Number: orb1527819
• Category: Antibodies
• Description: PHF8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 650 and 700
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PHF8
• UniProt ID: Q9UPP1
• NCBI: CAI41576.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: ZNF422; PHD finger protein 8; MRXSSD; KDM7B; jumon
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 µg) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody orb1527819 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human PHF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHF8 antibody orb1527819 used for IP at 6 µg per reaction.

Detection of human PHF8 by immunohistochemistry. Samples: FFPE serial sections of human small cell lung cancer. Antibody: Affinity purified rabbit anti-PHF8 (Cat. No. orb1527819) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB

Detection of mouse PHF8 by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-PHF8 (Cat. No. orb1527819) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB