PHF16 Antibody

• Catalog Number: orb1524462
• Category: Antibodies
• Description: PHF16 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 773 and 823
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PHF16
• UniProt ID: Q92613
• NCBI: NP_055550.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PHD finger protein 16; PHF16; JADE-3; jade family
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PHF16 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HeLa, HEK293T, and SW620 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF16 antibody (orb1524462) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human PHF16 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHF16 antibody (orb1524462) used for IP at 6 µg per reaction. PHF16 was also immunoprecipitated by a previous lot of this antibody (orb1524462) and a second antibody against a different epitope of PHF16.