PGM3 monoclonal antibody (M01), clone 1E2-1B12

• Catalog Number: orb2292414
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant PGM3.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1E2-1B12
• Tested applications: ELISA, IHC-P, IP, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: PGM3 (AAH01258, 1 a.a. ~ 542 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHLDHVMFRMGLLAVLRSKQTKSTIGVMVTASHNPEEDNGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRVLIDISEKEAVNLQQDAFVVIGRDTRPSSEKLSQSVIDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRYGKATIEGYYQKLSKAFVELTKQASCSGDEYRSLKVDCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKLNHLCGADFVKSHQKPPQGMEIKSNERCCSFDGDADRIVYYYHDADGHFHLIDGDKIATLISSFLKELLVEIGESLNIGVVQTAYANGSSTRYLEEVMKVPVYCTKTGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF
• NCBI: AAH01258
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged PGM3 is approximately 3 ng/ml as a capture antibody.

Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2 ug/ml]

Immunoprecipitation of PGM3 transfected lysate using anti-PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 MaxPab rabbit polyclonal antibody.

PGM3 monoclonal antibody (M01), clone 1E2-1B12 Western Blot analysis of PGM3 expression in HeLa.

Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12. Lane 1: PGM3 transfected lysate (59.9 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody (M01), clone 1E2-1B12 (Cat # orb2292414). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (85.36 KDa).