PGAM5 Antibody

• Catalog Number: orb1239864
• Category: Antibodies
• Description: PGAM5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Gorilla, Monkey, Primate
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-PGAM5 antibody (orb1239864) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5. The immunogen is located within the last 50 amino acids of PGAM5.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5 μg/mL; IF: 10-20 μg/mL. Antibody validated: Western Blot in human, mouse, and rat samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 32kD Observed: 32 kD
• Target: PGAM5
• UniProt ID: Q96HS1
• NCBI: Q96HS1
• Storage: PGAM5 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PGAM5 Antibody is supplied in PBS containing 0.02% sodium azide
• Alternative names: Serine/threonine-protein phosphatase PGAM5 mitocho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PGAM5, orb1239864 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of PGAM5 in Human Liver. Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with orb1239864 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PGAM5 in Mouse Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with orb1239864 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PGAM5 in Rat Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with orb1239864 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).