PFKFB4 Antibody (Center)

• Catalog Number: orb1928549
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB04059
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:100, IHC-P: 1:250
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 54040 Da
• Target: This PFKFB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-296 amino acids from the Central region of human PFKFB4.
• UniProt ID: Q16877
• NCBI: NP_004558.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphata
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Staining PFKFB4 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining PFKFB4 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/100) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-PFKFB4 Antibody (Center) at 1:1000 dilution. Lane 1: Raji, whole cell lysate. Lane 2: Molt-4 whole cell lysate. Lane 3: NCI-H1299 whole cell lysate. Lane 4: 293 whole cell lysate. Lane 5: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.