PEX3 Antibody

• Catalog Number: orb1238074
• Category: Antibodies
• Description: PEX3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine, Gallus, Monkey
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-PEX3 antibody (orb1238074) was raised against a peptide corresponding to 14 amino acids near the center of human PEX3. The immunogen is located within the center of PEX3.
• Concentration: 1 mg/mL
• Dilution range: WB: 2 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 42kDObserved: 42 kD
• Target: PEX3
• UniProt ID: P56589
• NCBI: P56589
• Storage: PEX3 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PEX3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PEX3 Antibody: Peroxisomal biogenesis factor 3, Pe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Validation of PEX3 in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with orb1238074 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

WB Validation in Human Cell Lines. Loading: 15 µg of lysate. Antibodies: PEX3 orb1238074, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX3 orb1238074, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX3 orb1238074, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of PEX3 in Human HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with orb1238074 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX3 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with orb1238074 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX3 in Rat testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with orb1238074 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).