PEX10 Antibody

• Catalog Number: orb1238073
• Category: Antibodies
• Description: PEX10 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Equine, Gorilla, Monkey, Primate
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-PEX10 antibody (orb1238073) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10. The immunogen is located within last 50 amino acids of PEX10.
• Concentration: 1 mg/mL
• Dilution range: WB: 4 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 37kD Observed: 37 kD
• Target: PEX10
• UniProt ID: O60683
• NCBI: O60683
• Storage: PEX10 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PEX10 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PEX10 Antibody: Peroxin-10, Peroxisomal biogenesis
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

WB Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysate. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of PEX10 in Human Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX10 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX10 in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX10 in Rat Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).