Peroxiredoxin 6/PRDX6 Antibody

• Catalog Number: orb251533
• Category: Antibodies
• Description: Peroxiredoxin 6/PRDX6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224). Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunocytochemistry , 0.5-1μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 25035 MW
• UniProt ID: P30041
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Peroxiredoxin-6;1.11.1.15;1-Cys peroxiredoxin;1-Cy
• Note: For research use only
• Application notes: WB: The detection limit for Peroxiredoxin 6 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

ICC analysis of PRDX6 using anti-PRDX6 antibody.PRDX6 was detected in immunocytochemical section of PC-3 cell.Biotinylated goat anti-rabbit IgG was used as secondary antibody.

IHC(P) analysis of Human Mammary Cancer Tissue using Anti-Peroxiredoxin 6 Picoband antibody.

WB analysis of Peroxiredoxin 6 using anti-Peroxiredoxin 6 antibody.Lane 1:human placenta tissue;2:human PC-3 cell;3:human K562 cell;4:human HeLa cell;5:rat lung tissue;6:rat liver tissue;7:mouse lung tissue;8:mouse liver tissue.

IHC analysis of PRDX6 using anti-PRDX6 antibody.PRDX6 was detected in paraffin-embedded section of human gastric cancer tissues.

IHC analysis of PRDX6 using anti-PRDX6 antibody.PRDX6 was detected in paraffin-embedded section of human Lung cancer tissues.

IHC analysis of PRDX6 using anti-PRDX6 antibody.PRDX6 was detected in paraffin-embedded section of human Ovarian cancer tissues.

IHC analysis of PRDX6 using anti-PRDX6 antibody.PRDX6 was detected in paraffin-embedded section of rat brain tissues.