Peroxiredoxin 5/PRDX5 Antibody

• Catalog Number: orb251567
• Category: Antibodies
• Description: Peroxiredoxin 5/PRDX5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198). Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22086 MW
• UniProt ID: P30044
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Peroxiredoxin-5, mitochondrial;1.11.1.15;Alu corep
• Note: For research use only
• Application notes: WB: The detection limit for Peroxiredoxin 5 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC(P) analysis of Mouse Intestine Tissue using Anti-Peroxiredoxin 5 Picoband antibody.

IHC(P) analysis of Rat Intestine Tissue using Anti-Peroxiredoxin 5 Picoband antibody.

IHC(P) analysis of Human Lung Cancer Tissue using Anti-Peroxiredoxin 5 Picoband antibody.

Flow Cytometry analysis of A549 cells using anti-Peroxiredoxin 5 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Western blot analysis using Anti-Peroxiredoxin 5 Picoband antibody.Lane 1:A549 Cell;2:Rat Brain Tissue;3:Mouse Brain Tissue.

IF analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody. Peroxiredoxin 5 was detected in immunocytochemical section of HELA cell.

IHC analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody.Peroxiredoxin 5 was detected in immunocytochemical section of SMMC Cell.