Peroxiredoxin 3/PRDX3 Antibody

• Catalog Number: orb251532
• Category: Antibodies
• Description: Peroxiredoxin 3/PRDX3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Peroxiredoxin 3 recombinant protein (Position: T110-Q256). Human Peroxiredoxin 3 shares 93% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 27693 MW
• UniProt ID: P30048
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Thioredoxin-dependent peroxide reductase, mitochon
• Note: For research use only
• Application notes: WB: The detection limit for Peroxiredoxin 3 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-Peroxiredoxin 3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody.Lane 1:Rat Brain Tissue;2:Mouse Brain Tissue;3:Rat Skeletal Muscle Tissue;4:Mouse Skeletal Muscle Tissue;5:U20S Cell;6:HELA Cell;7:SMMC Cell;8:RH35 Cell;9:A549 Cell.

IF analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody. Peroxiredoxin 3 was detected in immunocytochemical section of U20S cells.

IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody. Peroxiredoxin 3 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody.Peroxiredoxin 3 was detected in immunocytochemical section of HeLa cell.

IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody.Peroxiredoxin 3 was detected in immunocytochemical section of SMMC-7721 cell.

IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody.Peroxiredoxin 3 was detected in immunocytochemical section of MCF-7 cell.