HSPG2 Antibody

SKU: orb1252524

Description

HSPG2 Antibody

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Bovine, Fish, Human, Monkey, Mouse, Porcine
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Perlecan antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: A7L6
• Immunogen: A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.
• Target: HSPG2
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Perlecan antibody (A7L6).

SDS-PAGE Analysis of Purified, BSA-Free Perlecan Antibody (clone A7L6). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

HSPG2

UniProt

P98160