PERK antibody

• Catalog Number: orb338886
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EIF2AK3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PERK. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EIF2AK3
• Entrez: 9451, 29702
• UniProt ID: Q9Z2B5, Q9NZJ5, Q9Z1Z1
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PEK antibody, anti PERK antibody, anti Eukary
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Dai, Liying et al. Angiopoietin-2 silence alleviates lipopolysaccharide-induced inflammation, barrier dysfunction and endoplasmic reticulum stress of intestinal epithelial cells by blocking Notch signaling pathway Bioengineered, (2021)

PubMed: 34565297

Western blot analysis of PERK expression in A549 (A), MCF7 (B), 3T3 (C) whole cell lysates. (Predicted band size: 125 kD; Observed band size: 125 kD)

Immunohistochemical analysis of PERK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.