Periostin Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1227217
• Category: Antibodies
• Description: Periostin Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 2F7
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Periostin
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:20-50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 100 kDa
• Target: POSTN
• UniProt ID: Q15063
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: OSF 2; OSF2; osteoblast specific factor 2(fascicli
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Periostin was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (orb1227217, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody, red).

Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Periostin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1227217, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western blot analysis of Periostin on human brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST in PBS for 1 hour at room temperature. The primary antibody (orb1227217, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200000 dilution was used for 1 hour at room temperature. Predicted band size: 93 kDa, Observed band size: 80 kDa.