Pericentrin/Kendrin Antibody

• Catalog Number: orb1528567
• Category: Antibodies
• Description: Pericentrin/Kendrin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IP, WB
• Reactivity: Human
• Immunogen: between 175 and 225
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; ICC-IF - 1:1,000 - 1:5,000. Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Pericentrin/Kendrin
• UniProt ID: O95613
• NCBI: NP_006022.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: pericentrin; PCTN2; SCKL4; pericentrin-B; pericent
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Pericentrin-Kendrin by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Hep-G2, and K-562 cells. Antibody: Affinity purified rabbit anti-Pericentrin-Kendrin antibody at 0.04 µg/ml.

Detection of human Pericentrin-Kendrin by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-Pericentrin-Kendrin antibody.

ICC: Sample: HeLa cells. Antibody: Rabbit anti-Pericentrin/Kendrin at 1:1, 000. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated. Counterstain. DAPI (blue) and Phalloidin (green).