Perforin/PRF1 Antibody

• Catalog Number: orb215909
• Category: Antibodies
• Description: Perforin/PRF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Perforin recombinant protein (Position: E175-W555). Human Perforin shares 68% amino acid (aa) sequence identity with both mouse and rat Perforin.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 61377 MW
• UniProt ID: P14222
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Perforin-1;P1;Cytolysin;Lymphocyte pore-forming pr
• Note: For research use only
• Application notes: WB: The detection limit for Perforin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Perforin using anti-Perforin antibody.Lane 1:recombinant human Perforin protein 0.5 ng.

WB analysis of Perforin using anti-Perforin antibody.Lane 1:human HeLa cell; 2:human COLO320 cell; 3:human HepG2 cell.