Perforin antibody (PE)

• Catalog Number: orb222762
• Category: Antibodies
• Description: Mouse monoclonal antibody against Perforin conjugated to PE
• Clonality: Monoclonal
• Clone Number: dG9
• Tested applications: FC
• Reactivity: Bovine, Human
• Isotype: Mouse IgG2b kappa
• Immunogen: purified granules from human YT lymphoma cell line
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: Perforin
• Entrez: 5551
• UniProt ID: P14222
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-PRF1 antibody, anti-P1 antibody, anti-PFP ant
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Expression profiling on peripheral blood subsets using Anti-human Perforin PE antibody (clone dG9). Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm buffer set. HCDM CDMaps standardized procedures were used for leukocyte isolation. Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, EXCELLYSE XPerm buffer set was used for leukocytes permeabilization according to the instruction procedure. Mouse monoclonal anti-human Perforin PE antibody (clone dG9) was used in concentration 10 µg/ml in stained blood sample (2 x 10^6 cells).

Anti-human Perforin PE antibody (clone dG9) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm solution. Mouse monoclonal anti-human Perforin PE antibody (clone dG9) was used in concentration 10 µg/ml in stained blood sample (2 x 10^6 cells).