Perforin antibody

• Catalog Number: orb222761
• Category: Antibodies
• Description: Mouse monoclonal antibody to Perforin
• Clonality: Monoclonal
• Clone Number: dG9
• Tested applications: FC, ICC, IHC-Fr, IHC-P, IP
• Reactivity: Bovine, Human
• Isotype: Mouse IgG2b kappa
• Immunogen: purified granules from human YT lymphoma cell line
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 3-12 μg/ml. Intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Perforin
• Entrez: 5551
• UniProt ID: P14222
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-PRF1 antibody, anti-P1 antibody, anti-PFP ant
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 3-12 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of perforin positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using anti-human perforin (dG9) purified antibody (concentration in sample 2 μg/ml, GAM FITC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Perforin (dG9) purified antibody (concentration in sample 2 μg/ml, GAM FITC).