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Pepsin Antibody Peroxidase Conjugated
Catalog Number: orb344365
| Catalog Number | orb344365 |
|---|---|
| Category | Antibodies |
| Description | Pepsin antibody (Peroxidase) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | DOT, ELISA, IHC, WB |
| Reactivity | Porcine |
| Isotype | IgG |
| Immunogen | Pepsin [Porcine Stomach] |
| Antibody Type | Primary Antibody |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:1,000 - 1:5,000, IHC: 1:125 - 1:625, WB: 1:200 - 1:2,500 |
| Form/Appearance | Lyophilized |
| Purity | Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined. |
| Conjugation | HRP |
| UniProt ID | P00791 |
| NCBI | AAA31095.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | goat anti-Pepsin Antibody HRP Conjugated, Peroxida Read more... |
| Note | For research use only |
| Application notes | Anti-Pepsin peroxidase has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Peroxidase conjugated Goat anti-Pepsin antibody. Lane 1: Pepsin. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1000 for 60 min at RT Block: orb348637 for 30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Other band(s): none.
