Pepsin Antibody Biotin Conjugated

• Catalog Number: orb344381
• Category: Antibodies
• Description: Pepsin antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: Pepsin [Porcine Stomach]
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:10,000, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: Anti-Pepsin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]. Cross reactivity against Pepsin from other sources may occur but have not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P00791
• NCBI: AAA31095.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Pepsin Antibody Biotin Conjugation, Biot
• Note: For research use only
• Application notes: Anti-Pepsin biotin antibody has been tested by ELISA and western. This product is suitable for Immunohistochemistry. Expect band at 41.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western Blot of Goat anti-Pepsin Antibody Biotin Conjugated. Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody Biotin Conjugated 1:1000 overnight at 4°C. Secondary antibody: HRP Streptavidin (p/n orb348767) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 41 kDa, 41 kDa for Pepsin.