PENK antibody

• Catalog Number: orb19671
• Category: Antibodies
• Description: Goat polyclonal antibody to PENK
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 30.8
• Target: Proenkephalin
• Entrez: 5179
• Protein Sequence: RSHHQDGSDNEE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti proenkephalin antibody, anti PENK antibody
• Note: For research use only
• Application notes: IHC: Frozen section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus. Recommended concentration, 0.3-1μg/ml.WB: Approx 37kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrinology. 1998 Apr;139(4):2137-45; PMID: 9529003). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human adrenal gland lysate (35µg protein in RIPA buffer) using Proenkephalin antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human hypothalamus tissue using Proenkephalin antibody