PEF1 Antibody

• Catalog Number: orb546391
• Category: Antibodies
• Description: PEF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PEF1 recombinant protein (Position: R170-L284).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 30 kDa
• UniProt ID: Q9UBV8
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Peflin; PEF protein with a long N-terminal hydroph
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of PEF1 using anti-PEF1 antibody.Lane 1:human HEK293 cell;2:human placenta tissue;3:human PC-3 cell;4:human U-87MG cell;5:human U-937 cell;6:human HepG2 cell;7;human A431 cell;8;human U2OS cell.

WB analysis of PEF1 using anti-PEF1 antibody.Lane 1:rat lung tissues lysates;2:rat testicular tissue;3:mouse lung tissue;4:mouse testicular tissue.

IHC analysis of PEF1 using anti-PEF1 antibody.PEF1 was detected in paraffin-embedded section of mouse testis tissues.

IHC analysis of PEF1 using anti-PEF1 antibody.PEF1 was detected in paraffin-embedded section of rat testis tissues.

IHC analysis of PEF1 using anti-PEF1 antibody.PEF1 was detected in paraffin-embedded section of human colon cancer tissues.

IHC analysis of PEF1 using anti-PEF1 antibody.PEF1 was detected in paraffin-embedded section of human lung cancer tissues.