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Catalog Number | orb1538061 |
---|---|
Category | Antibodies |
Description | PECAM-1 / CD31 Antibody (clone 2F7B2) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2F7B2 |
Tested applications | ELISA, IF, IHC, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Purified recombinant fragment of human CD31 expressed in E. Coli. |
Concentration | 1 mg/ml |
Dilution range | ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200), WB (1:100 - 1:500) |
Conjugation | Unconjugated |
Target | PECAM-1 / CD31 |
Storage | Short term: store at 4°C. Long term: store at -20°C. |
Buffer/Preservatives | Ascitic fluid, 0.03% sodium azide. Ascitic fluid, 0.03% sodium azide |
Alternative names | PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM- Read more... |
Note | For research use only |
Application notes | Further information: Immunohistochemistry: LS-B3446 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3446 was determined to be 1:200. |
Expiration Date | 12 months from date of receipt. |
ELISA, IHC, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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