PDZ-GEF1 Antibody

• Catalog Number: orb1527469
• Category: Antibodies
• Description: PDZ-GEF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 975 and 1025
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PDZ-GEF1
• UniProt ID: Q9Y4G8
• NCBI: NP_055062.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: RAGEF; RA-GEF; RA-GEF-1; Rap guanine nucleotide ex
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PDZ-GEF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibody orb1527469 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody orb1527469 used for IP at 6 µg per reaction.

Detection of human PDZ-GEF1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PDZ-GEF1 (Cat. No. orb1527469) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB