PDLIM7 monoclonal antibody (M01), clone 2D6

• Catalog Number: orb2291745
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant PDLIM7.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: PDLIM7 (AAH01093.1, 1 a.a. ~ 457 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MDSFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVIRGRYLVALGHAYHPEEFVCSQCGKVLEEGGFFEEKGAIFCPPCYDVRYAPSCAKCKKKITGEIMHALKMTWHVHCFTCAACKTPIRNRAFYMEEGVPYCERDYEKMFGTKCHGCDFKIDAGDRFLEALGFSWHDTCFVCAICQINLEGKTFYSKKDRPLCKSHAFSHV
• Tested applications: ELISA, IF, IP, WB
• Clone Number: 2D6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH01093.1

Detection limit for recombinant GST tagged PDLIM7 is approximately 0.3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to PDLIM7 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoprecipitation of PDLIM7 transfected lysate using anti-PDLIM7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PDLIM7 MaxPab rabbit polyclonal antibody.

PDLIM7 monoclonal antibody (M01), clone 2D6 Western Blot analysis of PDLIM7 expression in Hela S3 NE.

Western Blot analysis of PDLIM7 expression in transfected 293T cell line by PDLIM7 monoclonal antibody (M01), clone 2D6. Lane 1: PDLIM7 transfected lysate (49.8 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of PDLIM7 over-expressed 293 cell line, cotransfected with PDLIM7 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDLIM7 monoclonal antibody (M01), clone 2D6 (Cat # orb2291745). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (76.01 KDa).