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Catalog Number | orb1227444 |
---|---|
Category | Assays and Kits |
Description | PDL2 Detection Set (Risk Free) |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | ELISA, FC, ICC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Mouse monoclonal PD-L2 antibodies were raised against the extracellular domain of human PD-L2. |
Concentration | Antibody 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | Antibodies can be stored at 4˚°C for three months and -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | PBS containing 0.02% sodium azide. |
Note | For research use only |
Expiration Date | Please enquire. |
Western blot analysis of PD-L2 in overexpressing 293 cells using orb1239853, orb1239856, orb1239859, orb1238786, and orb1239860 antibody at 0.5 and 1 μg/ml, respectively. Larger molecular weight bands represent more highly post-translationally modified PD-L2.
Immunocytochemistry of PD-L2 in overexpressing 293 cells using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 5 μg/ml.
Immunofluorescence of PD-L2 in overexpressing 293 cells using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 20 μg/ml.
Immunohistochemistry of PD-L2 in human colon carcinoma tissue using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 2 μg/ml.
Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.
Titration curve analysis of PD-L2 mAbs to detect recombinant PD-L2 in ELISA with orb1239853, orb1239856, orb1239859, orb1238786, and orb1239860 abs at decreasing concentrations.
Immunofluorescence of PD-L2 in human tonsil tissue using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 20 μg/ml.
Immunohistochemistry of PD-L2 in human tonsil tissue using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 2 μg/ml.