PDK4 antibody

• Catalog Number: orb1429323
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PDK4.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB03041
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: IF: 1:100, WB: 1:2000, WB: 1:2000, WB: 1:2000, IHC-P: 1:10~50, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 46469
• Target: PDK4
• UniProt ID: Q16654
• NCBI: NP_002603.1
• Alternative names: [Pyruvate dehydrogenase (acetyl-transferring)] kin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of HeLa cells stained with PDK4 (C-term) antibody at 1:100 dilution.Secondary antibody was Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green).Nuclei were counterstained with Hoechst 33342 (blue).

Western blot:Anti-PDK4 Antibody (C-term) at 1:2000 dilution + human heart lysate.Secondary antibody was Goat Anti-Rabbit IgG, (H+L).

Western blot:All lanes:Anti-PDK4 Antibody (C-term) at 1:2000 dilution.Lane 1:human heart lysate;Lane 2:K562.

Western blot:All lanes:Anti-PDK4 Antibody（C396）at 1:2000 dilution.Lane 1:human heart lysates;Lane 2:mouse skeletal muscle lysates.

Immunohistochemical analysis of human skeletal muscle reacted with PDK4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Orb1429323 staining PDK4 in human heart tissue sections by immunohistochemistry at 1:25 dilution.A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.