PDIA6 Antibody (Center K159)

• Catalog Number: orb1788326
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human PDIA6.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: 144-172 aa
• UniProt ID: Q15084
• MW: 48121 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:100, WB: 1:1000, IHC-P: 1:100, IHC-P: 1:100, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PDIA6; ERP5; P5; TXNDC7; Protein disulfide-isomera
• Note: For research use only
• NCBI: NP_005733.1

Flow cytometric analysis of hela cells using PDIA6 Antibody (Center K159) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis of paraffin-embedded H. brain section using PDIA6 Antibody (Center K159). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. liver section using PDIA6 Antibody (Center K159). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HT-1080, K562 cell line, human liver tissue, HepG2, C6 cell line (from left to right), using PDIA6 Antibody (Center K159). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center K159), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent image of HepG2 cells stained with XAF1 PDIA6 Antibody (Center K159). Diluted at 1: 100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).