PDIA2 Antibody

• Catalog Number: orb1249297
• Category: Antibodies
• Description: PDIA2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-EYKSTRDLETFSK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Human, Mouse and Rat Pancreas lysates (calculated MW of 58.2kDa according to NP_006840.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Pancreas shows ER staining in the beta cells of Islets of Lagerhans . Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PDIA2
• UniProt ID: Q13087
• NCBI: NP_006840.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PDIA2, protein disulfide isomerase family A, membe
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Human, Mouse and Rat Pancreas lysates (calculated MW of 58.2kDa according to NP_006840.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Pancreas shows ER staining in the beta cells of Islets of Lagerhans . Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249297 (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Pancreas lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249297 (3.8 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.