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Catalog Number | orb1958482 |
---|---|
Category | Proteins |
Description | PDGFRA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.7 kDa and the accession number is P16234-1. |
Tag | C-His |
Purity | SDS-PAGE: 96.4%; SEC-HPLC: 99% |
MW | 57.7 kDa (predicted); 70-80 kDa (reducing condition, due to glycosylation) |
UniProt ID | P16234 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | PDGFRA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.7 kDa and the accession number is P16234-1. |
Expression Region | A DNA sequence encoding the extracellular domain (Met 1-Glu 524) of human PDGFRα (NP_006197.1) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 24 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 57.4 kDa after removal of the signal peptide. The apparent molecular mass of PDGFRA-His is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result. |
98.00% | |
59.4 kDa (predicted) |
98.00% | |
59.2 kDa (predicted). Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result. |