PDGFRA Antibody / Platelet Derived Growth Factor Receptor alpha

SKU: orb1823456

Description

Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-a and -beta, which are endowed with protein tyrosine kinase domains. PDGFR-a can bind to both A and B subunits of PDGF, while PDGFR-beta can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two a receptors, PDGF-AB induces dimerization of aa and abeta and PDGF-BB induces the formation of three types of dimers, aa, abeta and betabeta. Translocation of the PDGFR-beta gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the PDGFRA antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: PDGFRA/7407
• Immunogen: A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the PDGFRA antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human colon cancer tissue with PDGFRA antibody (clone PDGFRA/7407). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PDGFRA antibody (clone PDGFRA/7407) as confirmation of integrity and purity.

Quick Database Links

UniProt

P16234