PDE8a antibody

• Catalog Number: orb154411
• Category: Antibodies
• Description: Mouse monoclonal antibody to PDE8a which is a member of the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8B is expressed specifically and abundantly in the thyroid gland and shares 65% sequence identity with PDE8A. It highly expressed in testis, ovary, small intestine and colon. It may be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.
• Clonality: Monoclonal
• Clone Number: EM-52
• Tested applications: WB
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: Human PDE8a
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: PDE8A
• Entrez: 5151
• UniProt ID: O60658
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HsT19550 antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western bloting analysis of human PDE8A using mouse monoclonal antibody EM-52 on lysates of HeLa cell line and TF-1 cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-PDE8A followed by Alexa Fluor® 790-conjugated goat-anti-mouse secondary antibody. A specific band was detected for PDE8A at approximately 95 kDa.