PDE4D Antibody

• Catalog Number: orb1254864
• Category: Antibodies
• Description: PDE4D Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 249-518 of human PDE4D (NP_001184152.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 110kDa
• Target: PDE4D
• UniProt ID: Q08499
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PDE4D, phosphodiesterase 4D, cAMP-specific (phosph
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PDE4D antibody (orb1254864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of HeLa cells using PDE4D antibody (orb1254864). Blue: DAPI for nuclear staining.