PD1 antibody (APC)

• Catalog Number: orb443267
• Category: Antibodies
• Description: Mouse monoclonal antibody against PD1 conjugated to APC
• Clonality: Monoclonal
• Clone Number: EH12.2H7
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1 kappa
• Immunogen: recombinant human CD279
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: CD279
• Entrez: 5133
• UniProt ID: Q15116
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-PDCD1 antibody, anti-PD1 antibody, anti-SLEB2
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human CD297 positive cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD279 (EH12.2H7) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD279 (EH12.2H7) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).