PDCD1 Antibody

SKU: orb1239734

Description

PDCD1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  PD-1 antibody can be used for detection of PD-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Flow cytometry at 0.1 μg/ml.Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 10B3
• Immunogen: PD-1 antibody was raised against the extracellular domain of human PD-1.
• Target: PDCD1
• Molecular Weight: Predicted: 32 kDa Observed: 38 kDa
• Purification: PD-1 Antibody is supplied as protein A purified IgG1.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 [10B3] antibody at 10 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.

(A) Immunohistochemistry of PD-1 in human spleen tissue with PD-1 [10B3] antibody at 10 μg/mL. (B) Immunohistochemistry in human spleen tissue with control mouse IgG staining at 10 μg/mL.

Immunofluorescence of PD-1 in human spleen tissue with PD-1 [10B3] antibody at 10 μg/mL. Green: PD1 Antibody [10B3] (orb1239734) Blue: DAPI staining

Flow cytometry analysis of PD-1 transfected 293 cells using PD-1 [10B3] antibody at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.

(A) Immunohistochemistry of PD-1 in human breast cancer tissue with PD-1 [10B3] antibody at 10 μg/mL. (B) Immunohistochemistry in human breast cancer tissue with control mouse IgG staining at 10 μg/mL.

Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA with orb1239745, orb1239769, orb1239763, orb1239738, and orb1239734 abs at decreasing concentrations.

Quick Database Links

Gene Symbol

PDCD1

UniProt

Q15116

RefSeq (Protein):NP_005009