PD-L1 Mouse Monoclonal Antibody

SKU: orb1499407

Description

PD-L1 Mouse Monoclonal Antibody

Research Area

Autoimmune, Cancer Research, Cell Markers, Immunology

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1ug/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 3B11
• Immunogen: Recombinant human PD-L1 protein (15-239/290aa)
• Target: CD274
• Molecular Weight: 29 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Programmed cell death 1 ligand 1; CD274; B7 H; B7 H1; B7 homolog 1; B7-H1; B7H; B7H1; CD 274; CD274 antigen; CD274 molecule; MGC142294; MGC142296; OTTHUMP00000021029; PD L1; PD1L1_HUMAN; PD1L1_Mouse; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL 1; PDL1; Programmed death ligand 1; RGD1566211.

Blank control (black line): MDA-MB-231. Primary Antibody (green line): Mouse Anti-Human PD-L1 antibody (orb1499407), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Human MDA-MB-231 cell lysates, Primary: Anti-Human PD-L1 (orb1499407) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 29 kDa, Observed band size: 53 kDa.

Quick Database Links

Gene Symbol

CD274

UniProt

Q9NZQ7