PD-1 Antibody [RMP1-14], Rabbit IgG

• Catalog Number: orb1671853
• Category: Antibodies
• Description: PD-1 Antibody [RMP1-14], Rabbit IgG
• Clonality: Recombinant
• Clone Number: RMP1-14
• Tested applications: Blocking, FC, IHC-Fr
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: The original rat hybridoma was generated by immunizing Sprague Dawley rats with mouse PD-1-transfectked BHK cells. A P3U1 myeloma was used as the fusion partner.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD279, PD1, PDCD1, RMPI-14, RMP1/14, Programmed De
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using anti-PD-1 antibody RMP1-14. Staining of murine splenocytes (unstimulated and 3-day Con A stimulated) with the rmeric version of RMP1-14 (orb1671853) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody. Con A stimulation induces PD-1 expression as evident by the increased fluorescence signal.

Immunofluorescence staining of fixed mouse splenocytes with anti-PD-1 (programmed cell death protein 1) antibody RMP1-14. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of RMP1-14 (orb1671853) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671853- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.