PCSK9 (C Terminus) Antibody

• Catalog Number: orb18975
• Category: Antibodies
• Description: Goat polyclonal antibody to PCSK9
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• Target: PCSK9 (C Terminus)
• Protein Sequence: CRSRHLAQASQELQ
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PCSK9; FH3; NARC1; HCHOLA3; proprotein convertase
• Note: For research use only
• Application notes: WB: Approx 75kDa band observed in Human Adipose lysates (calculated MW of 74.3kDa according to NP_777596.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1;. IHC: Paraffin embedded Human Liver. Recommended concentration: 5-6µg/ml.
• Expiration Date: 12 months from date of receipt.

orb18975 staining (0.2ug/ml) of McA cell lysates: untransfected (1), transfected with wild type human pcsk9 (2), transfected with S127R human pcsk9 (3). Data kindly provided by Dr. X-M Sun, Hammersmith Hospital, London.

orb18975 (0.3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18975 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. This data is from a previous batch.