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Catalog Number | orb1788053 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Target | This PCSK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the N-terminal region of human PCSK9. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Immunogen | 144-173 aa |
UniProt ID | Q8NBP7 |
MW | 74286 Da |
Tested applications | FC, WB |
Dilution range | WB: 1:1000, WB: 1:1000, FC: 1:25, FC: 1:25 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Proprotein convertase subtilisin/kexin type 9, 342 Read more... |
Note | For research use only |
NCBI | NP_777596.2 |
Flow cytometric analysis of HeLa cells using PCSK9 Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Flow cytometric analysis of A431 cells using PCSK9 Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
All lanes: Anti-PCSK9 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: HepG2 whole cell lysates. Lane 3: SW480 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 74 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-PCSK9 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: HepG2 whole cell lysates. Lane 3: SW480 whole cell lysates. Lane 4: human plasma lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 74 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.