pCp-Desthiobiotin

SKU: orb533108

Description

pCp-Desthiobiotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Desthiobiotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Desthiobiotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Desthiobiotin moiety can be performed using streptavidin con-jugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Desthiobiotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.

Images & Validation

• Application Notes:
  Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase. ; Spectroscopic Propertie: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 698.60 g/mol (free acid); Detected MW: 698.24 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₂₅H₄₄N₆O₁₃P₂
• SMILES: Nc1nc(=O)n(cc1)[C@@H]1O[C@H](COP(=O)(O)O)[C@H]([C@H]1O)OP(=O)(OCCCCCCNC(=O)CCCCC[C@H]1C(NC(=O)N1)C)O

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only