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Catalog Number | orb533108 |
---|---|
Category | Tools |
Description | pCp-Desthiobiotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Desthiobiotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Desthiobiotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Desthiobiotin moiety can be performed using streptavidin con-jugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Desthiobiotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads. |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | solution in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 698.60 g/mol (free acid); Detected MW: 698.24 g/mol (free acid) |
SMILES | Nc1nc(=O)n(cc1)[C@@H]1O[C@H](COP(=O)(O)O)[C@H]([C@H]1O)OP(=O)(OCCCCCCNC(=O)CCCCC[C@H]1C(NC(=O)N1)C)O |
Formula | C25H44N6O13P2 |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase.
Spectroscopic Propertie: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
Expiration Date | 12 months from date of receipt. |
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