PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

• Catalog Number: orb2320292
• Category: Antibodies
• Description: PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PC10
• Tested applications: FC, IHC, WB
• Reactivity: Drosophila, Gallus, Human, Monkey, Mouse, Porcine, Rat, Yeast, Zebrafish
• Isotype: IgG2a
• Immunogen: Rat PCNA/protein A fusion protein
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 36kDa
• Target: PCNA
• Entrez: 5111
• UniProt ID: P12004
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cyclin; DNA polymerase delta auxiliary protein; Mu
• Note: For research use only
• Application notes: Positive Control: A549, Tonsil or reactive lymph node.
• Expiration Date: 12 months from date of receipt.