PCNA Antibody

• Catalog Number: orb2639044
• Category: Antibodies
• Description: Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCNA/694
• Tested applications: FACS, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full length human protein was used as the immunogen for the PCNA antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This PCNA antibody is available for research use only.
• UniProt ID: P12004
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PAX-7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of PFA-fixed human HeLa cells with PCNA antibody (clone PCNA/694); Red = isotype control, Blue = PCNA antibody.

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (clone PCNA/694).

Western blot analysis of HepG2 cell lysate using PCNA antibody (clone PCNA/694). Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.