PCNA Antibody

• Catalog Number: orb1824134
• Category: Antibodies
• Description: Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCNA/4540
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human PCNA protein was used as the immunogen for the PCNA antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This PCNA antibody is available for research use only.
• UniProt ID: P12004
• Storage: Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human Raji cell lysate with PCNA antibody (clone PCNA/4540). Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.

IHC staining of FFPE human tonsil tissue with PCNA antibody (clone PCNA/4540) at 2 ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PCNA antibody (clone PCNA/4540) as confirmation of integrity and purity.