PCMT1 antibody

• Catalog Number: orb341147
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PCMT1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human PCMT1
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PCMT1
• Entrez: 5110
• UniProt ID: P23506, P22061
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Protein-L-isoaspartate(D-aspartate) O-methyltransf
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PCMT1 expression in HepG2 (A), A431 (B), mouse liver (C), rat brain (D), rat heart (E) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)

Immunofluorescent analysis of PCMT1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.